FYGVE Practical Pathology 2015-2016
Session 2
Instructions:
Click on the slide to view it. Manipulating the image should be self-explanatory. You will just need to be able to move around on the slide and click on the different objectives for this exercise. The options at the top of the screen can be ignored.
For each slide:
- Classify the sample as adequate or not. Specifically, are there enough cells, are they spread out in a monolayer, did they stain well? Most or all of these are adequate, but this is what you should ask yourself each time you review or submit a cytology sample.
- Describe the material on the slide.
- Give a diagnosis or a short list of differential diagnoses.
- What would you do next?
Case 1: Fine-needle aspirate from a firm, subcutaneous mass on dorsum of 3 year old, intact male, German shepherd. Handler thinks it has been present a couple of weeks, does not seem to bother the dog.
Case 2: Fine-needle aspirate from a 'small mass' in the right hip region of an 11 year old, MN, Siamese mix. Owner noticed incidentally while picking up the cat, and is not sure how long it has been present.
Case 3: Fine-needle aspirate from a 3.5 cm, firm, subcutaneous mass on the left lateral stifle from a 7 year old, FS, Beagle. Had been present at least 4 months, and the owner does not think it has changed in size.
Case 4: Fine-needle aspirate from a 3 cm, irregular, deep cervical mass, displacing the trachea, 15 year old, female, mixed breed dog.
Case 5: Fine-needle aspirate, right mandibular node, 8 year old, MN, Labrador. The veterinarian noticed an enlarged node on physical exam during a routine visit.
Case 6: Blood smear from a 4 year old, male, Great Dane. The owner said the dog is lethargic, doesn't really want to move.
Case 7: Fine-needle aspirate from a 0.1 cm mass on a digit from the left hind limb of a 5 year old, female, mixed breed dog.
Case 8: Direct smear of abdominal fluid from a 12 year old mare collected 1 day after colic surgery.
Case 9: Direct smear of pericardial effusion taken from a 13 year old, neutered male, Golden Retriever.
Case 10: Smear of tissue obtained from a bladder wall mass, 8 year old, spayed female, mixed breed dog. This dog has had intermittent lower urinary signs and multiple UTIs in the past 6 months.
NOTE: The slide scanner does not allow magnification equivalent to what you would see using oil immersion (100X). I picked cases here where I thought you could 'see' enough at 40X to reasonably assess the slide. When you do cytology in-house, you should use your oil objective lens. If you don't use oil, you run the risk of missing important, potentially diagnostic, details.